The great utilization of agricultural residues poses a persistent world problem. Trichoderma reesei is a conventional cellulase and different protein producer utilizing agricultural residues as substrates. The potential of T. reesei as a chassis to supply small pure merchandise stays untapped. Right here, we efficiently employed T. reesei to effectively synthesize terpenes from a number of sources. To optimize the chassis for metabolite synthesis, we deleted main (hemi-)cellulase genes together with the worldwide regulator Lae1, to enhance the effectivity of secondary metabolite biosynthesis, and overexpressed the constitutively activated transcriptional issue XYR1A824V in MC3 to derepress glucose repression. By way of the refined sugars and corn steep as substrates, the manufacturing of ophiobolin F utilizing the modified chassis was elevated to 1187.06 mg/L in shake flask fermentation and as much as 3072.45 mg/L below fed-batch fermentation. We additional demonstrated the flexibility of the Δlae1::xyr1/MC3-Δ10 chassis by efficiently producing different fungal and plant terpenes. Collectively, our outcomes demonstrated the potential of the Decreasing Outflow and Broadened Upstream Substrate Kind (ROBUST) T. reesei chassis for environment friendly terpene manufacturing using agricultural residues, with essential implications for terpene biosynthesis and sustainable biofabrication.