Lignin valorization is indispensable for a inexperienced biorefinery. Enzymatic depolymerization utilizing ligninolytic enzymes, like manganese and lignin peroxidases, is a promising method. Nevertheless, enzymatic depolymerization carried out in a batch system is hindered by a repolymerization response. Right here, we efficiently enhanced the lignin depolymerization effectivity by performing peroxidase-catalyzed depolymerization of beech wooden lignin in a just lately reported membrane bioreactor, wherein water-soluble lignin fragments are constantly handed by way of a membrane. The entire quantity of the water-soluble lignin fragments utilizing the membrane bioreactor turned out to be maximally 28-fold increased than that with a batch bioreactor. GC-MS evaluation confirmed the presence of a wide range of quick aliphatic and fragrant compounds as constituents of the water-soluble lignin fragments. Moreover, lignin quantification of and SEC analyses of the remaining strong residue within the membrane bioreactor indicated a better diploma of lignin depolymerization and elimination. Semi-quantitative NMR evaluation additionally supported the efficient lignin elimination within the membrane bioreactor. These findings exhibit the effectiveness of the membrane bioreactor for the enhancement of native lignin depolymerization and elimination by peroxidases.