Microalgae-based carbon sequestration is rising as a inexperienced and sustainable method to obtain detrimental carbon whereas recycling CO₂ into biomass used for the manufacturing of bioenergy and value-added merchandise. Nonetheless, its profitable implementation continues to be to be realized because of the low solubility of CO₂ and ion accumulation with the addition of bicarbonate within the tradition medium. On this research, we proposed, developed and verified a novel system integrating electrolysis and ionic membranes (EIMs) that permits the synthetic recycling of CO₂ utilization and alleviation of steel cation stress in microalgae cultivation. HCO₃⁻ was chosen to switch from the cathode chamber to the tradition pond with sodium bicarbonate because the catholyte, whereas Na⁺ cations had been blocked with the anionic membrane in EIMs, accompanied by a regularly reducing pH worth, which facilitates microalgae development. The reliability and universality of EIMs was additional verified with each a cation-tolerant marine pressure, Dunaliella salina HTBS, and cation-sensitive freshwater strains, Chlamydomonas and Chlorella. Specifically, the cell densities of cation-sensitive strains in EIMs had been a lot greater than these within the NaHCO₃ group in each 800 mL- and 150 L-scale functions, demonstrating their nice potential. Furthermore, the intracellular metabolites weren’t affected when microalgae had been cultured in EIMs, implying their feasibility for business cultivation. Subsequently, we established strong EIMs that facilitate each the environment friendly utilization of CO₂ and business software, which can make clear the event of inexperienced know-how for microalgae-based carbon sequestration sooner or later.