Hydrothermal liquefaction (HTL) presents a promising method for the conversion of moist waste into biocrude and biofuels. Nonetheless, the post-hydrothermal liquefaction wastewater (PHW) poses important challenges for therapy and valorization as a consequence of its excessive focus and sophisticated nature. On this research, we investigated the conversion pathways of main natural contaminants throughout the microbial electrolytic therapy of PHW from meals waste HTL. To realize this, we employed high-performance liquid chromatography (HPLC) and 2D nuclear magnetic resonance (NMR). Our findings display unstable fatty acids (VFAs) and monohydric and polyhydric alcohols had been successfully reworked by way of the synergistic metabolism of fermentative and electroactive micro organism, which led to over 70% COD removing of the recalcitrant compounds and a report excessive H2 manufacturing charge (1.62 L L-1 d-1). We additionally employed the liquid-state 15N NMR on wastewater samples for the primary time and revealed that the nitrogen-containing heteroaromatics had been persistent to microbial electrochemical therapy. By integrating the chemical profiles with bioanode neighborhood profiles, we constructed a metabolic community that gives insights for enhancing therapy effectivity and facilitating useful resource restoration.