Home Biology Zebrahub: Illuminating Zebrafish Embryogenesis By Gentle-Sheet Microscopy and scRNA Sequencing.

Zebrahub: Illuminating Zebrafish Embryogenesis By Gentle-Sheet Microscopy and scRNA Sequencing.

Zebrahub: Illuminating Zebrafish Embryogenesis By Gentle-Sheet Microscopy and scRNA Sequencing.


Gentle-sheet time-lapse of Tg(h2afva:h2afva-mcherry ; mezzo:eGFP) embryo beginning at 50% epiboly.

What’s Zebrahub?

Zebrahub is a web-based platform that gives an built-in strategy to finding out and analyzing mobile lineages throughout zebrafish embryogenesis at each transcriptional and spatiotemporal ranges. It combines single-cell sequencing time course knowledge with lineage reconstructions facilitated by light-sheet microscopy. The scRNAseq gene expression throughout tissues and time factors might be instantly visually explored in an online browser.  Moreover, researchers can navigate the complicated mobile flows and lineages derived from light-sheet microscopy knowledge utilizing Zebrahub’s on-line internet viewer and desktop interactive device, enabling exploration of complete timelapse datasets and digital destiny mapping experiments.

What impressed the event of Zebrahub?

Once we initiated the undertaking, it was clear that the neighborhood was lacking a database combining systematic live-imaging-based cell lineages with single-cell sequencing throughout growth. Historically these approaches have been used individually, thereby limiting the total understanding of growth. We needed to make use of probably the most superior microscopy and sequencing strategies accessible to ascertain a multimodal baseline of zebrafish growth.

How can scientists make the most of Zebrahub of their analysis?

Many vital discoveries in developmental biology originated from observations; due to this fact, from the start, we needed to have a completely interactive web site to empower scientists by making our knowledge accessible and, extra importantly, instantly explorable, even to scientists with out coding abilities.  Thus, regarding our terabyte-scale high-resolution light-sheet imaging time-lapse dataset, we offer each an neuroglancer-based interactive viewer for total multi-color time-lapse datasets and a napari plugin to carry out in silico destiny mapping experiments.  Equally, we enable researchers to effortlessly seek for particular gene expressions and the emergence of cell varieties utilizing CZI’s cell-by-gene platform. Our dedication to accessibility led us to take a position substantial effort in making certain that our knowledge, code, and blueprints are available and open supply. By doing so, we anticipate a direct affect on a wide-ranging neighborhood, spanning disciplines resembling developmental biology, single-cell biology, superior microscopy, laptop imaginative and prescient, tissue morphodynamics, and computational biology.

Who’re the individuals behind this useful resource?

People behind this resource

Supported by the CZ Biohub San Francisco (CZB-SF), Zebrahub is led by Merlin Lange,  senior scientist within the lab of Loic Royer, and outcomes from an interdisciplinary collaboration with CZB’s Information Science and Genomics Platforms, with key contributors amongst others: Angela Oliveira Pisco, Norma Neff, and Alejandro Granados. Total, Zebrahub has greater than 30 collaborators at CZB-SF collaborating establishments (see image above). Many are early-career scientists who performed a key function within the undertaking. Within the following, these younger scientists introduce themselves and their contributions:

Shruthi : I’m Shruthi VijayKumar, a analysis affiliate within the Royer lab. A big a part of my function concerned creating and optimizing the one embryo single cell dissociation protocol for the assorted developmental levels in zebrahub together with Michael Borja (Genomics) and Merlin Lange. Moreover, I contributed to making ready the libraries required for sequencing and helped with cell annotations. This undertaking has been an unbelievable expertise, because it has supplied me with the chance to work in a multidisciplinary and collaborative atmosphere. This expertise has not solely given me a greater understanding of all the pipeline but in addition broadened my experience by means of intensive interactions with totally different groups. The truth that Zebrahub is an increasing useful resource for the neighborhood makes this undertaking much more particular and rewarding to be part of.

Sarah: My identify is Sarah Ancheta, and I’m an affiliate Information Scientist. As a part of the Zebrahub workforce, I labored on knowledge processing and evaluation of inter-individual transcriptomic variability. Since Zebrahub has single-cell transcriptomic datasets from individually resolved embryos, I used to be capable of dissect the embryo-to-embryo variability by creating a framework to analyze the variations in gene expression between sibling people over time. I really feel lucky to work with knowledge of such top quality and backbone and excited to have been among the many first to investigate Zebrahub’s knowledge and discover new organic insights into zebrafish growth. It has been an exquisite expertise to work with such an interdisciplinary workforce and study to conduct analyses from each a organic and data-driven perspective.

Mike: My identify is Michael Borja, and I’m a Senior Analysis Affiliate for the Genomics platform. My important function and accountability is to supervise lab actions relating to Single Cell and Spatial Transcriptomic experiments. When Zebrahub initially began, I used to be tasked with choosing which single-cell transcriptomic assay to make use of for our preliminary experiments. From there, I led the early efforts of processing cell dissociations and library preparation of particular person embryos. As well as and in partnership with Shruthi VijayKumar, we developed a sturdy zebrafish single-cell dissociation protocol from single embryos that’s impartial of the developmental stage. Being a part of this multidisciplinary and modern workforce has helped me see how large-scale tasks throughout the realm of science might be idealized and accomplished. As I proceed with my private progress and path within the discipline of Genomics, I’ll completely proceed to hunt large-scale collaborations resembling Zebrahub in order that I might be able to apply what I’ve discovered by means of this expertise. 

Jordao: My identify is Jordao Bragantini. I’m a software program engineer within the Royer lab. My main focus revolves round picture processing, cell segmentation, and monitoring. The Zebrahub undertaking uncovered me to the fascinating world of terabyte-scale microscopy pictures in addition to to the mysteries of Developmental Biology. Dealing with this huge quantity of knowledge acquired on our superior light-sheet microscopes required implementing specialised algorithms, starting from GPU-friendly approximate morphological operators to our personal distributed monitoring software program. These analyses require the total capability of our accessible computational sources. I couldn’t have wished for a greater solution to have interaction with machine studying within the biology area than working with this multidisciplinary workforce, the place we will study from one another’s experience.

What are the following steps for Zebrahub?

We need to increase Zebrahub with extra developmental levels, novel multiomic modalities, and extra lineage-specific imaging to finally reconstruct a digital multimodal embryo. We’re additionally engaged on integrating current datasets, together with these from various species, to create a complete atlas of vertebrate embryogenesis. This bold endeavor heralds a transformative period for the fields of developmental and evolutionary biology.

You may learn extra about Zebrahub within the BioRxiv preprint.

NMP centered RNA velocity UMAP (static)

Thumbs up (No Scores But)



Please enter your comment!
Please enter your name here